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Titration of the in vitro binding of wild-type, M46A, and N49A mutant VAMP-2 to GST–syntaxin 1A and GST–SNAP-25. Shown are in vitro binding of wild-type and mutant VAMP-2 at the indicated concentrations to (A) immobilized GST–syntaxin 1A (0.3 μm) and (B) immobilized GST–SNAP-25 (0.3 μm). Bound VAMP-2 was recovered and visualized by Western blotting, as described in Materials and Methods.
