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. 1997 Mar 1;17(5):1596–1603. doi: 10.1523/JNEUROSCI.17-05-01596.1997

Fig. 4.

Fig. 4.

Potentiation of wild-type and Δ51–60 mutant VAMP-2 binding to GST–syntaxin 1A in the presence of soluble SNAP-25.In vitro binding of wild-type (A) and Δ51–60 mutant VAMP-2 (B) at the indicated concentrations to immobilized GST–syntaxin 1A (0.3 μm), immobilized GST–SNAP-25 (0.3 μm), and immobilized GST–syntaxin 1A in the presence of soluble SNAP-25 (1 μm). C, In vitro binding of wild-type VAMP-2 at the indicated concentrations to immobilized GST–SNAP-25 (ΔC) (0.3 μm) and immobilized GST–syntaxin 1A (0.3 μm) in the presence of soluble SNAP-25 (ΔC) (1 μm). Bound VAMP-2 was recovered and visualized by Western blotting, as described in Materials and Methods.