Fig. 6.

Formation of heat-labile, SDS-resistant ternary complexes with wild-type and mutant forms of VAMP-2. Combinations of soluble wild-type or mutant VAMP-2, syntaxin 1A, and SNAP-25 (2 μm each) were incubated for 16 hr at 4°C. After SDS-PAGE sample buffer was added, samples were divided into two aliquots and either incubated at 37°C (a) or boiled (b). After SDS-PAGE and transfer to nitrocellulose, multimeric complexes were detected by using an antibody against syntaxin 1A and visualized by autoradiography. Binary controls are shown in the three pairs of lanes at the far left. The molecular weight (MW) markers are in kDa.