Table 1.
Analysis of in vivo interactions between wild-type or mutant VAMP-2 and syntaxin 1A or SNAP-25 with the yeast two-hybrid system
| VAMP-2 “bait” | Syntaxin 1A “prey” | SNAP-25 “prey” |
|---|---|---|
| Wild-type | + | ++ |
| Δ2–30 | − | − |
| Δ31–40 | − | + |
| Δ41–50 | − | − |
| Δ51–60 | − | − |
| Δ61–70 | − | + |
| Δ71–80 | − | − |
| M46A | + | + |
| N49A | ++ | +++ |
Two-hybrid constructs were prepared and cotransfected into the reporter strain SFY526, as described in Materials and Methods. β-Galactosidase activity was monitored by using X-gal as a substrate and is expressed as either a + (blue colonies) or − (white colonies) signal. The number of +’s reflects the intensity of the blue color.