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Changes in [Ca]C caffeine are different from those of the calcium stores, measured in permeabilized cells. A–C, Control conditions. A, Baseline; B, response to a pulse of caffeine (10 mm, 300 msec); C, 3 min later. The cell was permeabilized for 2–3 min with intracellular medium containing 10 μm digitonin and ATP. [Ca]i was clamped by EGTA at 150 nm. D–F, Same sequence of exposure to caffeine, 5 min after the cell was permeabilized. Contrast-enhanced images.
