Fig. 1.

Melatonin prevents death of N2a exposed to Aβ(25–35). N2a cells were plated and after 24 hr, during exponential growth phase, were treated with either scrambled peptide (control), adriamycin (control for apoptotic cell death; Marin et al., 1996), 50 μm Aβ(25–35), or 50 μm Aβ(25–35) with 10 μm melatonin for an additional 24 hr. Live cells were assessed by their fluorescence with BODIPY green (also see Fig. 2,right panels). Results are reported as means ± SD of four experiments (2 duplicate experiments on different days; minimum 100 cells studied per plate). *Measurements significantly different from control (p ≤ 0.02, pairedt test).