Fig. 6.

Explant cultures were treated with 150 μm DRB for 0, 0.5, 2, 4, or 8 hr and processed for ISHH, using an LHRH probe. Top panel, LHRH mRNA levels per cell (O.D./cell) were measured, values from slices 2–5 were pooled, and means ± SE of each treatment group were plotted to calculate LHRH mRNA turnover. Bottom panel, For all control and batch II slice explants, all discernible LHRH mRNA-containing cells were counted in slices 2–5; values were meaned within slices and treatment groups; means from slices within a treatment group were summed, the SE propagated, and the mean cell number per whole animal ±SE for each group calculated and plotted to determine LHRH mRNA turnover. DRB had a significant effect on cell number (p < 0.001, ANOVA), and the zero point cell number was significantly different from that of each DRB time point (Bonferroni’s Multiple Comparison Test, p < 0.05).