Table 1.

DRB treatment reduces LHRH mRNA levels in single cells as determined by ISHH

DRB (hr)	Median O.D. units
0	16130  (1628, 62)
0.5	11003  (280, 8)a
2	10125  (213, 36)a
4	10796  (98, 41)a
8	5891  (324, 50)a,b,c,d

Three days before experimentation, 1 μmtetrodotoxin was added to culture medium. On day 18 of culture and in the continued presence of tetrodotoxin, explant cultures were treated with vehicle (0 hr) or 150 μm DRB for 0.5, 2, 4, or 8 hr and then processed for ISHH. After hybridization, cultures were dipped in NTB3 autoradiography emulsion and developed after 21 d. LHRH mRNA levels per cell (O.D./cell) were measured, and median values (n cells, n cultures) for each time point are shown. A nonlinear curve was fit to the data, using a two-phase exponential decay equation. Similar to that observed for the mean O.D. data, the initial rate constant and t_1/2 are 8.07 hr⁻¹ and 0.086 hr, respectively.

^a p < 0.001 from 0 hr, using the Kolmogorov–Smirnov Two-Sample Test.

^b p < 0.001 from 0.5 hr, using the Kolmogorov–Smirnov Two-Sample Test.

^c p < 0.001 from 2 hr, using the Kolmogorov–Smirnov Two-Sample Test.

^d p < 0.001 from 4 hr, using the Kolmogorov–Smirnov Two-Sample Test.