Table 2.

Transcription inhibitor treatment does not reduce LHRH cell number as determined by immunocytochemistry^a

Slice	Control	DRB 4 hr	DRB 16 hr
2	11.6  ± 3.1	26.8  ± 11.7	15.2  ± 6.3
	(7)	(4)	(6)
3	33.5  ± 8.8	28.8  ± 10.6	27.6  ± 7.9
	(12)	(6)	(11)
4	30.6  ± 3.7	39.0  ± 10.6	29.7  ± 6.4
	(15)	(7)	(10)
5	12.7  ± 4.0	15.3  ± 4.4	6.9  ± 2.3
	(11)	(4)	(9)
Whole animal	88.4  ± 10.8	109.9  ± 19.5	79.4  ± 12.2

^F2-a Two days before experimentation, 1 μm tetrodotoxin was added to culture medium. On day 18 of culture and in the continued presence of tetrodotoxin, slices were treated with 150 μm DRB or vehicle (Control) for times as indicated, fixed for immunocytochemistry, and stained for LHRH immunoreactivity. Data are mean ± SE counts of LHRH-positive cells per culture (n). Within slice or whole animal analyses, no significant differences between treatment groups were detected (p > 0.05, ANOVA).