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Western blot analysis of nuclear proteins. To determine the size of c-Fos protein, Western blot analysis was performed using tissue extracts of cingulate cortex from rats after 6 hr of enforced wakefulness (SD1, SD2), and followed by 1 hr of sleep (RS1, RS2) before death. c-Fos protein (molecular weight, 62 kDa; arrowhead) decreased in rats that were allowed sleep before death.
