Fig. 2.
5-HT1B receptor mRNA in trigeminal ganglia. Total RNA isolated from day 14 neonatal rat trigeminal ganglia and brain and from CA77 cells was reverse-transcribed and PCR-amplified, using 5-HT1B specific primers. A 303 bp 5-HT1B cDNA product was detected in CA77 cells (lane 3), trigeminal ganglia (lane 5), and rat brain (lane 7). As controls, RT-PCR was performed with H2O as a substitution for an RNA template (lane 2) or in the absence of reverse transcriptase (lanes 4, 6, 8). A 100 bp DNA standard is shown in lane 1. An inverse image of an ethidium bromide-stained gel is shown.