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CGS increases the concentration of intracellular calcium in primary trigeminal neuron cultures. Intracellular calcium concentrations, [Ca2+]i, were measured, using fura-2 in trigeminal neurons after 3 d in culture.A, Basal levels in cells loaded for 45 min.B, Levels 4 min after the addition of 10 μm CGS. Marked increases in [Ca2+]i are observed in the soma of trigeminal neurons. The pseudocolor scale indicates the 340/380 nm excitation wavelength ratio and [Ca2+]i.
