Fig. 4.

CGS treatment downregulates CGRP steady-state mRNA levels. A, Northern blot analysis of total RNA (3 μg) from vehicle-treated (CON) or CGS-treated (CGS; 10 μm for 16 hr) CA77 cells. The filter was hybridized with cDNA probes for CGRP (top) or CO II (bottom). B, Levels of CGRP mRNA from CGS-treated cells relative to control cells. The CGRP signals were normalized to the density of the CO II band in the vehicle- and CGS-treated cells. The means and SE from four independent experiments are shown.