Table 2.
Agent | n | Basal (nm) | Peak (nm) | Fold increase | Peak time (sec) |
---|---|---|---|---|---|
CGS (1 μm) | 29 | 64 ± 3.0 | 139 ± 4.5 | 2.17 ± 0.08 | 355 ± 17 |
CGS (1 μm) + 0.1 μm MET | 25 | 61 ± 1.8 | 85 ± 3.1 | 1.41 ± 0.04 | 352 ± 13 |
CGS (1 μm) + 1.0 μm MET | 42 | 52 ± 2.2 | 61 ± 3.3 | 1.18 ± 0.05 | 368 ± 10 |
CGS (10 μm) | 40 | 66 ± 2.9 | 265 ± 18 | 4.02 ± 0.30 | 319 ± 16 |
CGS (10 μm) + 10 μm MET | 19 | 64 ± 4.4 | 142 ± 2.7 | 2.22 ± 0.04 | 325 ± 23 |
CGS (10 μm) + 50 μm MET | 32 | 70 ± 3.1 | 117 ± 3.8 | 1.67 ± 0.05 | 291 ± 13 |
CGS (10 μm) + 100 μm MET | 36 | 58 ± 1.9 | 69 ± 2.6 | 1.20 ± 0.04 | 305 ± 9 |
CGS (10 μm) + 2 μm SB | 26 | 51 ± 1.9 | 197 ± 9.8 | 3.89 ± 0.19 | 308 ± 19 |
CGS (10 μm) + 50 μm SB | 31 | 64 ± 2.8 | 274 ± 15 | 4.28 ± 0.27 | 341 ± 19 |
mCPP (50 μm) | 15 | 73 ± 5.4 | 263 ± 5.5 | 3.60 ± 0.08 | 293 ± 24 |
Sumatriptan (20 μm) | 26 | 60 ± 3.2 | 198 ± 6.8 | 3.30 ± 0.11 | 218 ± 18 |
CGS (10 μm) + PTX | 85 | 69 ± 3.0 | 280 ± 8.3 | 4.06 ± 0.11 | 328 ± 17 |
Sumatriptan (20 μm) + PTX | 27 | 55 ± 2.8 | 187 ± 7.3 | 3.40 ± 0.09 | 222 ± 19 |
The values are the mean and SE (n = number of cells). Basal values were measured in untreated cells 60 sec after the start of the experiment. Peak values are the maximum [Ca2+]i measured during the 10 min sampling period. The fold increase indicates the change from basal to peak calcium levels calculated for each individual cell. The peak times are the time of maximal recorded [Ca2+]i after the addition of the agent. Cells were preincubated with methiothepin (MET) or SB 206553 (SB) for 5 min before the addition of CGS or treated overnight with pertussis toxin (PTX, 100 ng/ml). Cells were loaded for 30 min with fura-2.