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. 1997 Dec 15;17(24):9520–9535. doi: 10.1523/JNEUROSCI.17-24-09520.1997

Fig. 9.

Fig. 9.

Affinity-purified anti-caveolin polyclonal antibody and anti-caveolin mAb 2234 recognize different spectrums of polypeptides by two-dimensional immunoblot analyses. Detergent-insoluble complexes isolated from type 1 astrocytes, MDCK cells, and lung tissue were separated by two-dimensional SDS-PAGE and processed for immunoblot analyses using either affinity-purified anti-caveolin polyclonal antibody (A, C, E) or anti-caveolin mAb 2234 (B, D, F). A complex antigen profile of apparent molecular mass between 22 and 31 kDa and isoelectric points ranging from pH ∼5 to 6.5 is detected by the polyclonal antibody in astrocytes (A), MDCK cells (C), and lung (E). A simplified antigen profile of apparent molecular mass between 22 and 31 kDa and of isoelectric points ranging from pH ∼5.5 to 6 is detected by mAb 2234 in astrocytes (B), MDCK cells (D), and lung (F). Molecular mass × 10−3 is indicatedvertically; pH values are indicatedhorizontally.