Table 1.

Sublethal oxygen–glucose deprivation (OGD) does not affect overall GluR-B mRNA abundance in rat hippocampal cultures containing both neurons and glia

AMPA receptor	Sham wash	OGD	p
GluR-A	47.5  ± 7.2	41.6  ± 9.4	0.623
GluR-B	25.9  ± 5.9	18.9  ± 6.5	0.434
GluR-C	17.2  ± 5.5	25.2  ± 3.7	0.240
GluR-D	9.4  ± 1.9	14.3  ± 4.5	0.330

Sister cultures were exposed to sublethal oxygen–glucose deprivation (20–30 min), allowed to recover for 24 hr, and extracted for RNA. RT-PCR products were quantitated using a phosphorimager, and the relative abundance of each transcript was expressed as percentage of total for each culture (mean ± SEM; n = 12 measurements; p was calculated using two-tailed Student’st test).