Table 3.

Sublethal oxygen–glucose deprivation (OGD) increases kainate-mediated excitotoxicity

Condition	Cell death (%)		p
	Sham wash	OGD
Blank	0  ± 1.8
30 μm kainate	38.4  ± 2.13-a	58.8  ± 4.4a,b	0.0002
150 μmNMDA	44.5  ± 4.13-a	36.4  ± 3.13-a	0.114
100 min OGD	59.5  ± 3.43-a	44.7  ± 1.43-a	0.007

Kainate or NMDA was applied at the indicated concentrations for 24 hr to cultures subjected to a 30 min sublethal oxygen–glucose deprivation or sham wash 24 hr before excitotoxin exposure. Toxin-specific cell death was estimated by measuring LDH efflux into the bathing medium at the end of the excitotoxin exposure, subtracting LDH efflux from sham-washed cultures, and scaling to the mean LDH value corresponding to near-complete neuronal death (100%, induced by 24 hr exposure to 500 μm NMDA) in sister cultures. Sublethal oxygen–glucose deprivation does not affect LDH release induced by 24 hr exposure to 500 μm NMDA. n = 12 cultures per condition, mean ± SEM.

^F3-a Difference from cell death in cultures exposed to media alone (Blank); p < 0.05 using one-way ANOVA with Bonferroni correction.

^F3-b Difference from cell death in corresponding sham wash-conditioned cultures; p = 0.0002, using one-way ANOVA.