Skip to main content
. 1997 Dec 15;17(24):9492–9505. doi: 10.1523/JNEUROSCI.17-24-09492.1997

Fig. 2.

Fig. 2.

High potassium increases the dendritic localization of BDNF mRNA in cultured hippocampal neurons.A, Whole-cell patch-clamp recordings, under current-clamp conditions, after isotonic depolarization with 10 mm (top trace) and 20 mm KCl (bottom trace). The arrows mark the onset of the depolarization. The arrowheads mark the last 3 min of a 30 min recording time. Calibration: 45 mV, 1 min.B, In situ hybridization on cultured hippocampal neurons with BDNF riboprobe, viewed with Nomarski optics.Small arrows mark the maximal distance at which a dendritic labeling was scored (MDDL). In the dendrites, staining appears granular (large arrows). C, D, Depolarization with 10 mm KCl for 3 hr increases the dendritic localization of BDNF mRNA. E, At higher magnification, after 3 hr depolarization in 10 mm KCl, intensely labeled granules are detected within varicosities at great distance from the cell body (large arrows). Scale bar (shown in E): 20 μm for B, C, D; 14 μm for E.