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. 1997 Dec 15;17(24):9492–9505. doi: 10.1523/JNEUROSCI.17-24-09492.1997

Fig. 8.

Fig. 8.

A short incubation in high potassium increases BDNF and TrkB protein levels in the dendritic compartment. Immunohistochemistry on cultured hippocampal neurons. Each picture represents the integration in a single projection of a series of five optical sections obtained with a confocal microscope, as described in Materials and Methods. A, Anti-BDNF immunostaining of control cultures. B, Anti-BDNF immunostaining after 10 mm KCl depolarization for 10 min. C, Anti-BDNF staining after a preincubation with nocodazole for 6 hr followed by 10 min of 10 mm KCl depolarization in the continuous presence of nocodazole. D, Staining with anti-TrkB antibody in control conditions. E, Staining with anti-TrkB antibodies after 10 min depolarization in 10 mm KCl. F, Anti-TrkB staining after a preincubation with nocodazole for 6 hr followed by 10 min of 10 mm KCl depolarization in the continuous presence of nocodazole. Scale bar (shown in F): 20 μm forA–F.