Fig. 3.

Dose dependence of neuronal survival in NGF. Duplicate wells containing neurons either alone (open bars) or in co-cultures with myocytes (black bars) were grown at varying concentrations of NGF. After 63 hr, a strip of neurons was counted across the tissue culture well for each condition. The average number of neurons remaining in the well was computed and plotted as a function of NGF concentration. In three independent experiments, 5 ng/ml NGF was sufficient to support neuronal survival. Error bars represent SDs.