Fig. 1.

Immunoblot analysis of rat hippocampus (A) and receptor-expressing cells (B). Crude membrane preparations from rat hippocampus and CHO cells expressing mGluR4a (4a), mGluR7a (7a), or mGluR7b (7b), or COS cells transfected with mGluR8 cDNA (8) were subjected to 7% SDS-PAGE and transferred onto PVDF filters.A, The filters with the hippocampus were reacted with antibody to pan mGluR1 (G18), mGluR1α (A52), mGluR2/3 (H12), mGluR2 (mG2Na-5), mGluR4a (K44), mGluR5 (G53), mGluR7a (G71), mGluR7b (K74), or mGluR8 (K88). Immunoreactive bands for mGluR7b were completely abolished by preadsorption of the antibody with the corresponding peptide (adsorbed). B, The filters with the receptor-expressing cells were reacted with the mGluR7a (G71), mGluR7b (K74), mGluR4a (K44), or mGluR8 (K88) antibody. Each of the immunoreactive products was absent in nontransfected cells (not shown). Positions of molecular mass markers (Bio-Rad) in kDa are indicated on the left.