Fig. 6.

Double-immunofluorescence study for mGluR7b, mGluR1α, and mGluR7a in the hilus and CA3 stratum lucidum. Fluorescence micrographs of sections double-immunolabeled for mGluR7b and mGluR1α (A, A’) or mGluR7b and mGluR7a (B, B’) were taken from identical fields of the hilus (A, A’) and CA3 (B, B’) under different filters. Punctate immunolabeling for mGluR7b (A, visualized with Texas Red) decorates mGluR1α-immunopositive interneurons (A’, visualized with fluorescein) in the hilus (Hi). In CA3, all profiles decorated with mGluR7b (B) are also decorated with mGluR7a immunoreactivity (B’, visualized with fluorescein) in stratum lucidum (Lu). Stratum radiatum (Ra) is immunopositive only for mGluR7a (B’). Arrows indicate cell bodies decorated with mGluR7a/b and labeled for mGluR1α immunoreactivity.Gr, Granule cell layer; Py, pyramidal cell layer. Scale bar, 30 μm.