Fig. 4.
SDS-PAGE and immunoblot analysis of δ1 (TC1)- and δ2 (TC2)-transfected cell membranes, organ of Corti (OC), and spiral ganglion (SG) using anti-δ1 (A,B) and anti-δ1/2 (C) antibodies. With both antibodies (B, C), a major immunoreactive band, which comigrates with the δ1-immunoreactive band, is seen in OC and SG in both rat and guinea pig as well as in membranes of cells transfected with δ1 cDNA. Arrowheads show the positions of prestained standards (from top to bottom): myosin (Mr = 203,000), phosphorylaseb (Mr = 105,000), BSA (Mr = 71,000), ovalbumin (Mr = 44,600), carbonic anhydrase (Mr = 28,000), β-lactoglobulin (Mr = 18,000), and lysozyme (Mr = 15,000).