Fig. 2.

Alcohol administered on proestrus results in luteinized unruptured follicle. Photomicrographs of ovarian follicles in animals killed on estrus after treatment with vehicle (top) or alcohol (bottom) at 8 A.M. and 12 P.M. on proestrus. Note that the largest follicles present in control females were smaller and less numerous than those observed in animals exposed to alcohol on proestrus. The large follicles present in alcohol-treated animals were characterized by luteinization (note the greater number of layers as well as the increase in the ratio of cytoplasm to nucleus) of the granulosa layers. Morphological findings, summarized in Table 1, confirm that animals do not ovulate after injection with alcohol on the afternoon of proestrus. Magnification, 300×.