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. 1997 Apr 1;17(7):2295–2313. doi: 10.1523/JNEUROSCI.17-07-02295.1997

Fig. 3.

Fig. 3.

Specificity of BDNF immunostaining.A–C, Tissue sections processed for immunohistochemistry with either the BDNF antibody omitted (A), the BDNF antibody solution preadsorbed against purified recombinant human BDNF (B), or the BDNF antibody solution preadsorbed against mouse β-NGF (C) (bright-field illumination). As seen in A and B, when the BDNF antibody was omitted or preadsorbed with BDNF protein, no specific staining was present. In C, preadsorbing against mouse β-NGF revealed a pattern of immunostaining that was identical to sections reacted with the BDNF antibody alone (see Fig. 1). Scale bar, 2 mm.