Fig. 6.

Co-localization of PMP22 and LAMP1 inTr^J Schwann cells. Fresh-frozen sections of adult normal (A–C) andTr^J/+ (D–F) sciatic nerves were double-immunolabeled with polyclonal anti-PMP22 (A,D) and monoclonal LAMP1 (B,E) antibodies. Nuclei were stained with Hoechst dye (C, F). In the normal nerve, PMP22 is found in myelin (A) and is absent from the nodes of Ranvier (A, arrows). In comparison, PMP22 accumulates in the cell bodies of some of the Schwann cells inTr^J/+ nerve (D,arrowheads). A low level of LAMP1-like immunoreactivity is found in the normal nerve (B, arrows), which is markedly increased in theTr^J/+ nerve (E). Several Schwann cells are identified in theTr^J/+ sample and show co-localization of PMP22 and LAMP1 (arrowheads in D andE, respectively). In 10-d-old heterozygousTr^J nerve, PMP22-like immunoreactivity can be detected in the Schwann cell membrane (G). Scale bar (shown in G): 50 μm.