Fig. 7.

The Tr^J mutation alters the normal distribution pattern of MAG and P0. Fresh-frozen sections of adult normal (A, D) andTr^J/+ (B,C, E) sciatic nerves were examined for the distribution of MAG (A, B) and P0 (D, E). The normal localization pattern of MAG is shown in A. Intracellular MAG-like immunoreactivity (B, arrowheads) is co-localized with LAMP1 (C, arrowheads) in Tr^J/+ nerves. In the normal nerve, P0 is found in compact myelin (D). Reduced levels of myelin-like P0 immunoreactivity are present inTr^J/+ nerves (E). A nerve sample processed without primary antibody incubation is shown inF. Scale bar (shown in F): 80 μm.