Fig. 5.

Characterization of G2027. The top panel describes the cross-reactivity of G2027 to various structurally related and unrelated peptides presented as a competition curve of percentage of total binding (%B/Bo) versus the logarithm of competing peptide concentrations. The middle panel presents the elution of standard rat (R) CGRP-α (□) and -β (▴) from the HPLC column as described in Materials and Methods compared with rat CGRP-α RIA standard (○). Peak immunoreactivity was observed during the 24 min fraction in each sample. The bottom panel demonstrates the elution profile of CGRP immunoreactivity from extracted spinal cord slices after perfusion at 34 (○), 37 (□), and 40°C (▵) as compared with standard rat CGRP-α (•).