Fig. 1.

Tissues innervated by the SCG differentially express isoforms of the type I PACAP-selective (PACAPR), VIP₁/PACAP (VIP1R) and VIP₂/PACAP (VIP2R) receptors. Total RNA from individual adult male rat pineal, sublingual, and submaxillary glands; iris; and cerebral blood vessels was reverse-transcribed, and the cDNA was amplified using primers flanking the insertion site of the alternative splice variants of the type I PACAP receptor or primers specific for each of the VIP/PACAP receptors (Table 1). The amplified products and a 100 bp DNA ladder were resolved on 1.6% agarose gels, stained with ethidium bromide, and visualized by UV illumination. The predicted sizes of the products are 303, 387, and 471 bp for the type I PACAP receptor variants containing neither, one, and both 84 bp cassettes, respectively, and 323 and 396 bp for the VIP₁/PACAP and VIP₂/PACAP receptors.