Fig. 5.

Sympathetic neurons express pro-PACAP mRNA. Total RNA from individual adult and neonatal SCGs, and sympathetic neuronal cultures (2 cm² wells) was reverse-transcribed, and the cDNA was amplified for 35 cycles using primers PCP1 and PCP2 (Table 1), which are specific for the rat neuronal pro-PACAP transcript. The identity of the neuronal pro-PACAP reverse transcription-PCR products was verified using sequence-specific hybridization. For product identification, the amplified products were transferred to a nylon membrane and hybridized with an internal oligonucleotide end-labeled with [γ³²P]-ATP. The predicted 606 bp product identified by gene-specific probe hybridization of the amplified products is indicated.