Fig. 6.

PACAP mRNA is localized to a subpopulation of sympathetic neurons in vivo. In situ hybridization histochemistry using antisense (A) and sense (B) digoxigenin-labeled PACAP riboprobes was performed on adult male SCG cryosections as described in Materials and Methods. After incubation with antidigoxigenin alkaline phosphatase, sections were processed with nitro blue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate as phosphatase substrates. Scale bar, 50 μm.