Fig. 8.

THDOC increases late openings of single channel currents gated by a brief pulse of GABA. A, Channel activity evoked by a 1 msec pulse of 1 mm GABA as indicated by the current above each trace in an outside-out patch excised from a granule neuron in a cerebellar slice. The inset shows a fraction of the single channel current activity on an expanded time scale. B, Same as in A except that GABA was coapplied with THDOC (1 μm). C, D, Illustrated are the ensemble averages derived from 30 applications as inA and B, respectively. Ensemble responses are shown fit to biexponential functions with an indication of the slow decay time constant. Corresponding calibration bars are shownunder the right corner of the traces.