Fig. 8.
Expression of lacZ in the hippocampus of heterozygous mice; X-gal staining of tissue from NPY+/− mice. A, X-gal staining in a whole-brain section through the hippocampal formation from a normal heterozygous mouse. B, X-gal staining in a whole-brain section through the hippocampal formation 24 hr after a kainic-acid induced seizure (60 mg/kg, i.p.). Note the increased X-gal staining in the stratum oriens region of CA3 (arrow) and particularly in the granule cell layer of the dentate gyrus (arrowhead) in this thick tissue section.C, High magnification of X-gal staining in the stratum oriens region of CA3 (1000-μm-thick tissue section) from a different heterozygous mouse. D, High magnification of X-gal staining in the stratum oriens region of CA3 (1000-μm-thick tissue section) 24 hr after a kainic acid-induced seizure. E, High magnification of X-gal staining in the dentate gyrus (1000-μm-thick tissue section) from a different heterozygous mouse.F, High magnification of X-gal staining in the dentate gyrus (1000-μm-thick tissue section) 24 hr after a kainic acid-induced seizure.