Fig. 9.
Facilitatory effect of astrocyte culture medium conditioned by a 24 hr exposure to 17β-estradiol (CM-17β-E2) on cAMP formation (A) and LHRH release (B) induced by the treatment of GT1–1 cells with the EP3-R and EP1-R agonist sulprostone. CM-17α-E2, Medium from astrocytes treated with 17α-estradiol. The GT1–1 cells were pretreated with the CMs for 3 hr before a 30 min exposure to sulprostone; *p < 0.05,CM-17β-E2-pretreated groups versus control groups pretreated with CM-17α-E2and the control group not exposed to sulprostone. In the case ofCM-17α-E2-pretreated cultures versus controls not challenged with sulprostone, *p < 0.05.