Fig. 2.

Kv1.3 coexpression decreases v-Src-induced protein tyrosine phosphorylation. HEK 293 cells were transfected with control, v-src, Kv1.3, or Kv1.3 together with v-src (cotransfection) vectors.A, Two days after transfection, cell lysates were prepared, and lysate proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phosphotyrosine (top) or anti-Src (bottom) antibody.B, The protein phosphotyrosine signal was quantified by densitometry, and values are expressed relative to the v-Srclane (n = 10; ***p ≤ 0.005, Student’s t test).C, Expressed Src protein was quantified by densitometry, and values are expressed relative to the v-Src lane(n = 10).