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. 1997 Dec 1;17(23):8964–8974. doi: 10.1523/JNEUROSCI.17-23-08964.1997

Fig. 4.

Fig. 4.

Coexpression of tyrosine mutant Kv1.3 channels decreases v-Src-induced protein tyrosine phosphorylation.A, HEK 293 cells were transfected with control vector; v-src vector; Kv1.3 vector; a vector encoding one of the mutant Kv1.3 channels YYY111–113FFF Kv1.3, Y137F Kv1.3, Y449F Kv1.3, or Y479F Kv1.3; or the v-src vector together with wild-type (+) or mutant Kv1.3 (−) vector (cotransfection). Cells were harvested 2 d after transfection, and cell lysates were used for SDS-PAGE Western blotting using anti-phosphotyrosine antibody. B, The protein phosphotyrosine signal was quantified by densitometry, and values are expressed relative to the v-Src lane (range ofn, 4–9; **p ≤ 0.01 and ***p ≤ 0.005, Student’s t test). The statistical comparison is between the wild-type channel and the various Y-to-F mutant constructs.