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. 1997 Dec 1;17(23):8964–8974. doi: 10.1523/JNEUROSCI.17-23-08964.1997

Fig. 5.

Fig. 5.

Kv channel gating influences the attenuation of v-Src-induced protein tyrosine phosphorylation. HEK 293 cells were transfected with control, v-src, Kv1.4, Kv1.5, Kv1.4 together with v-src, or Kv1.5 together with v-src vectors. A, Cell-attached patch recordings were made 2 d after transfection. Currents evoked by depolarizing voltage pulses to +40 mV are shown for Kv1.4 and Kv1.5. B, Two days after transfection, cell lysates were prepared, and lysate proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody. C, The protein phosphotyrosine signal was quantified by densitometry, and values are expressed relative to the v-Src lane (n = 6; ***p ≤ 0.005, Student’s ttest).