Fig. 2.
Northern blot analysis of mRNA prepared from different adult rat tissues and rat brain of different developmental stages [postnatal days 5 (P5), 10 (P10), 15 (P15), 20 (P20) and 25 (P25) and adult] using the p421.HB cDNA clone (Fig. 1) and a cyclophilin-specifc cDNA probe. Ten micrograms of total RNA were separated on a 1.2% formaldehyde gel and hybridized with 32P-labeled DNA fragments specific for p421.HB/PD-Iα and cyclophilin. For quantification Northern blots were analyzed by phosphorimaging techniques, and mRNA levels were normalized to cyclophilin. For the diagram at thebottom the mRNA level in adult brain was set to 100%.