Table 2.

Effects of conditioned medium on the properties of spontaneous synaptic currents

Conditions		Rise time (msec)	Decay time (msec)	Amplitude (pA)	Frequency (events/min)
Ctr. med.	Before	1.3  ± 0.2	10.0  ± 0.9	485  ± 89	10.0  ± 0.9
n = 7	After	1.5  ± 0.2	11.2  ± 0.6	458  ± 74	11.2  ± 0.6
	Ratio	1.13  ± 0.06	1.14  ± 0.07	0.98  ± 0.07	0.9  ± 0.1
Cond. med.	Before	1.2  ± 0.2	9.7  ± 1.0	518  ± 64	12.3  ± 3.9
n = 9	After	1.4  ± 0.3	11.1  ± 1.0	480  ± 61	31.8  ± 7.3*
	Ratio	1.08  ± 0.03	1.08  ± 0.03	0.95  ± 0.06	3.8  ± 1.0*
Cond. med.	Before	1.4  ± 0.2	12.4  ± 1.2	451  ± 75	9.6  ± 1.9
+TrkC-IgG	After	1.4  ± 0.2	11.6  ± 0.5	429  ± 82	9.4  ± 2.2
n = 7
	Ratio	0.99  ± 0.06	0.97  ± 0.06	0.94  ± 0.09	1.0  ± 0.1

Spontaneous synaptic currents (SSCs) were recorded from an innervated myocyte in 1-d-old nerve–muscle cultures. The mean values of SSC properties before and 10 min after bath application of control (Ctr. med.) and conditioned media (Cond. med.) for each synapse were calculated by the SCAN program. Only the recordings that have >50 SSC events were used for the analysis. The values for before and after bath application of media were obtained by averaging the mean values for each synapse. To obtain ratio values, ratios between the mean value after medium application and that before medium application for each synapse were calculated before averaging. All values represent mean ± SEM, and n refers to the number of synapses recorded. The data were subject to Student’s ttest.

^*p < 0.05.