Fig. 2.
Inhibition of antipyretic effect of α-MSHin vivo by SHU-9119. Rats were treated with LPS (25 μg/kg, i.p.) at time 0 (arrow) and received the indicated injectates intracerebroventricularly 30 min later (arrow). A, Time course of LPS-induced fever in the presence and absence of intracerebroventricular α-MSH (300 ng; 180 pmol) or α-MSH plus SHU-9119 (200 ng; 168 pmol). Numbers of animals in each group are indicated in parentheses.Symbols represent mean ± SEM. B, Data from A represented as area under the temperature-time response curves (mean ± SEM). Overall statistical significance of treatment effects isF(2,24) = 8, p = 0.002; statistical significance of between-group comparisons is indicated in the figure. Baseline (time 0) Tb values for the respective groups were (°C; mean ± SEM) LPS/NaCl (▪), 37.6 ± 0.1; LPS/α-MSH (•), 37.7 ± 0.1; LPS/(α-MSH+SHU-9119) (▴), 37.6 ± 0.1.