Table 1.

Primers for RT-PCR

Target mRNA	Forward (F) and reverse (R) primers	Expected product size (base pairs)	Reference
IL-1α	F: 5′-AAGTTTGTCATGAATGATTCCCTC-3′	263	Lomedico et al., 1984
	R: 5′-GTCTCACTACCTGTGATGAGT-3′
IL-1β	F: 5′-CAGGATGAGGACATGAGCACC-3′	447	Gray et al., 1986
	R: 5′-CTCTGCAGACTCAAACTCCAC-3′
TNF-α	F: 5′-ATGAGCACAGAAAGCATGATC-3′	276	Fransen et al., 1985
	R: 5′-TACAGGCTTGTCACTCGAATT-3′
IFN-γ	F: 5′-TACTGCCACGGCACAGTCATTGAA-3′ R: 5′-GCAGCGACTCCTTTTCCGCTTCCT-3′	405	Gray and Goeddel, 1983
CD3γ	F: 5′-ATGGAGCAGAGGAAGGGTCTG-3′	549	Renno et al., 1995
	R: 5′-TCACTTCTTCCTCAGTTGGTT-3′
β-actin	F: 5′-TGTGATGGTGGGAATGGGTCAG-3′	514	Alonso et al., 1986
	R: 5′-TTTGATGTCACGCACGATTTCC-3′