Fig. 3.

Catalytically active cdc2 is present in NFT-containing neurons in AD. Hippocampal tissue sections from AD (left panel), normal human (middle panel), and mouse brain (right panel) were stained as indicated (active kinase,top and bottom rows; inactive kinase,middle row), and light micrographs of representative cases are shown. The active kinase antibody stains AD neurons with NFT and some blood vessels in normal brain. Neurites in neuritic plaques (NP, bottom row) are also positive with the active kinase antibody. Some AD cases (bottom row) showed weaker staining of glial cells with the active kinase antibody in addition to the marked reactivity with NFT. None of the NFT in these cases stained positive with the inactive kinase antibody (bottom row, inactive), although blood vessels and glia were stained. Magnifications for the top andmiddle rows are 75×; for the NP, 400×; and for the remaining panels in the bottom row, 200×.