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. 1997 May 15;17(10):3710–3726. doi: 10.1523/JNEUROSCI.17-10-03710.1997

Fig. 5.

Fig. 5.

Glial environment in “mature cerebellar cultures.” A, Astrocytes immunostained with an antibody against GFAP. Bergmann fiber-like processes span the molecular layer (arrowheads). Notice the astrocytic plexuses in the granular layer and white matter at the central region of the folium. B, ED1-immunoreactive cells are present in large numbers all over the slice culture. C, Myelinated fibers, immunostained with an antibody against MBP, are present in white matter (wm) and in the granular cell layer but not in the molecular layer (ml). D, The CaBP-immunoreactive initial segments of Purkinje cell axons (arrowheads) enter the MBP-immunoreactive myelin sheaths (arrows), showing that the myelinated fibers belong to Purkinje cell axons. Scale bar: A, B, 40 μm;C, 80 μm; D, 20 μm.