Fig. 3.

Denatonium-induced changes in [Ca²⁺]_i depended on intracellular stores. This graph shows maximum denatonium-induced changes in [Ca²⁺]_i expressed as a percentage of resting [Ca²⁺]_i. Cells were tested twice, before (hatched bars) and during or after (open bars) the treatments indicated, as illustrated in Figure 2[Ca²⁺-free bath solution (0 Ca; n = 17), thapsigargin (n = 34), ryanodine (n = 12), and U73122 (n = 18)]. Control cells were tested twice, as illustrated in Figure1D (n = 16), the first and second stimulations indicated by the hatched andopen bars, respectively.