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Effect of GDP-β-S on the denatonium-induced outward current. This cell was held at −80 mV, and the membrane was stepped to +80 mV. TTX was present in the bath solution to block Na+ currents. A, After 6 min of whole-cell recording and B, after 15 min of whole-cell recording. Note that the GDP-β-S (1 mm in pipette solution) abolished the denatonium response, suggesting that the response was G-protein-dependent.
