Table 1.

Injections

Case	Target	Volume injected (nl)	Survival
1	Ce	200 PRV	48 hr
8	Ce	200 PRV	52 hr
26	Ce	200 PRV	48 hr
40	Ce	300 PRV	48 hr
46	Ce	200 PRV + 1% CTB	48 hr
47	Ce	200 PRV	26 hr
48	Ce	200 PRV	82 hr
49	Ce	200 PRV	48 hr
50	Ce	200 PRV	96 hr
51	Lateral PB	200 PRV + 1% CTB	96 hr
52	Lateral PB	200 PRV	75 hr
60	Ce	50 FG	7 d
61	Ce	40 FG	7 d
62	Ce	40 FG	7 d
67	Ce	40 FG	7 d
69	Lateral PB	50 PRV	120 hr
70	Ce	50 PRV	72 hr
71	Ce	40 FG (4%)	7 d + 72 hr
71	Ce	50 PRV	72 hr
85	Right VPM	200 PRV	76 min
85	Left VPM	200 PRV + 1 CTB	27 min
86	Left VPM	200 PRV + 1% CTB	66 min
86	Right VPM	200 PRV	22 min
87	Left VPM	100 PRV + 1% CTB	65 hr
88	Left VPM	100 PRV	66 hr
89	Left VPM	100 PRV	77 hr
91	Left VPM	100 PRV	72 hr
92	Left VPM	100 PRV	72 hr
93	Ce	100 PRV	52 hr
94	Ce	100 PRV	52 hr
95	Ce	100 PRV	52 hr
100	Lateral PB	40 CTB	7 d + 72 hr
100	Ce	100 PRV	72 hr
101	Lateral PB	40 CTB	7 d + 96 hr
101	Ce	100 PRV	96 hr
102	Lateral PB	40 CTB	7 d + 48 hr
102	Ce	100 PRV	48 hr
103	Lateral PB	80 PRV	56 hr
104	Lateral PB	80 PRV	72 hr
105	Lateral PB	80 PRV	72 hr
1501	Ce	200 PRV	90 hr
1508	Ce	200 PRV	94 hr
1510	Ce	200 PRV	78.5 hr

For all injections 20% of concentrated colloidal gold was added to each volume of PRV except when PRV was coinjected with CTB (cases 46, 85, 86, 87).

For cases 85, 86, 100, 101, and 102, FG or CTB were injected 7 d before PRV injection.

For cases 1501, 1508, and 1510, bilateral excitotoxic of the elPB were made 7 d before PRV injection.

Central nucleus of the amygdala (Ce); Cholera Toxin (CTB); Fluoro-Gold (FG); (PB) parabrachial; PRV, Bartha strain of pseudorables virus; VPM, ventroposteromedial nucleus of thalamus.