Table 2.

Contribution of AMPA and NMDA receptors to the glutamate-induced inhibition of protein synthesis

Treatment	[35S]Methionine incorporation (% of basal)
None	100  ± 7
MK-801 (2 μm)	112  ± 8
CNQX (100 μm)	94  ± 11
CNQX + MK-801	108  ± 11
GLU (100 μm)	54  ± 52-a
GLU + MK-801	71  ± 92-a
GLU + CNQX	59  ± 52-a
GLU + MK-801 + CNQX	91  ± 5

Neurons were exposed to antagonists for 1 min in Krebs’ bicarbonate buffer before a 5 min incubation period in the presence of [³⁵S]methionine (4 μCi/ml). [³⁵S]Methionine incorporation, expressed in the percentage of the mean of basal [³⁵S]methionine incorporation measured in the absence of any treatment (230,500 ± 17,000 dpm/mg protein, n = 3), was measured as indicated in the legend to Figure 6. None of these treatments altered significantly the amount of TCA-soluble [³⁵S]methionine, indicating that they did not modify methionine uptake into neurons. All values are the means ± SEM of data obtained in three experiments, each performed in triplicate on three different sets of cultured neurons.

^F2-aSignificantly different (p < 0.01) from basal [³⁵S]methionine incorporation (ANOVA, followed by Dunnett’s test).