Table 3.
Effects of protein translation inhibitors on NMDA receptor-mediated neurotoxicity
| Treatment | Neuronal survival (%) | ||
|---|---|---|---|
| None | Cycloheximide | Diphtheria toxin | |
| None | 100 | 100 ± 1 | 99 ± 2 |
| NMDA (10 μm) | 79 ± 4 | 96 ± 43-a | 100 ± 53-a |
| NMDA (100 μm) | 47 ± 2 | 55 ± 6 | 42 ± 1 |
Cortical neurons were exposed to the indicated concentrations of NMDA for 30 min. Cycloheximide (1 μg/ml) was added to cells for 30 min before, during, and for 2 hr after the NMDA incubation period. Diphtheria toxin (10 nm) was applied to cells 12 hr before the NMDA treatment. Neuronal survival was estimated 24 hr after the onset of NMDA application by measuring the rate of MTT reduction. Results, expressed in the percentage of the neuronal survival estimated in the absence of any treatment, are the means ± SEM of values obtained in three independent experiments, each performed in triplicate on different sets of cultured neurons.
Significantly different (p < 0.01) from the neuronal survival estimated 24 hr after a 10 μmNMDA treatment (ANOVA, followed by Student–Newman–Keuls test).