Fig. 5.

Elevation of phosphorylated c-Jun levels by glutamate, but not by dopamine or forskolin. A, Western blots probed with anti-phospho(Ser73)-c-Jun (top) and anti-c-Jun (bottom) antibodies performed on the same lysates of striatal cultures treated with or without glutamate (100 μm), dopamine (100 μm), or mannitol (300 mm) for the times indicated. B, Western blots probed as in A on lysates of striatal cultures treated with glutamate (100 μm) for the times indicated.C, Western blots probed with anti-phospho-CREB (top) and anti-CREB (bottom) antibodies performed on lysates of striatal cultures treated as inA. In A–C, the same blot was probed with antibodies directed toward the phosphorylated transcription factor and then reprobed with the antibodies directed toward the total form. The positions of flanking molecular weight markers are indicated on the right. All results were consistently reproducible in independent experiments.