Fig. 10.

APTRA-AM, a permeant chelator having a lower Ca²⁺ affinity and different structure from BAPTA-AM, is also effective. Slices were pretreated with 100 μm of either BAPTA-AM (11 slices) or APTRA-AM (9 slices). Controls were pretreated with DMSO alone (9 slices). Subsequently, the cultures were exposed to 60 min OGD. Neuronal survival at 3 and 5 hr after OGD was better in the chelator-treated groups (ANOVA on groups indicated in thedotted boxes was followed by Newman–Keuls procedure for multiple comparisons; p values indicate comparisons to chelator-untreated controls). A, Times 0–5 hr.B, Outcome at 24 hr.